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A monoclonal antibody to T‐2 toxin
Author(s) -
GOODBRAND I. A.,
STIMSON W. H.,
SMITH I. E.
Publication year - 1987
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1987.tb01623.x
Subject(s) - monoclonal antibody , toxin , microbiology and biotechnology , biology , virology , antibody , immunology
A specific, high affinity monoclonal antibody to T‐2 toxin of Fusarium has been produced. The monoclonal antibody was conjugated to horse‐radish peroxidase and employed to develop a direct competitive enzyme‐linked immunosorbent assay (ELISA) for the toxin. The sensitivity of the ELISA was 10 ng/ml with a working range up to 500 ng/ml. The antibody cross‐reacted with HT‐2 toxin (25%) but did not bind to any other trichothecene tested.