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Hydrophobic properties of Providencia stuartii and other Gram‐negative bacteria measured by hydrophobic interaction chromatography
Author(s) -
ISMAEEL N.,
FURR J. R.,
PUGH W. J.,
RUSSELL A. D.
Publication year - 1987
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1987.tb01622.x
Subject(s) - hydrophilic interaction chromatography , providencia , biofilm , escherichia coli , pseudomonas aeruginosa , bacteria , microbiology and biotechnology , mutant , strain (injury) , hydrophobic effect , biology , chemistry , adhesion , enterobacteriaceae , chromatography , biochemistry , high performance liquid chromatography , genetics , organic chemistry , anatomy , gene
A hydrophobic interaction chromatography (HIC) procedure was used to compare the relative surface hydrophobicity of three Providencia stuartii strains and wild type and envelope mutants of Escherichia coli and Pseudomonas aeruginosa. Providencia stuartii strain Pv2 adsorbed to a greater extent to octyl‐ and phenyl‐Sepharose than did Pv67. The HIC technique showed a significant difference in surface hydrophobicity between wild type and envelope mutant strains, the latter being considerably more hydrophobic. Pre‐treatment of cell suspensions with chlorhexidine produced further changes in the hydrophobic nature of all the strains. Moreover, HIC provides a convenient and rapid alternative means of screening strains for a property potentially associated with adhesiveness.