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A visual DNA probe for detection of enterotoxigenic Escherichia coli by colony hybridization
Author(s) -
ROMICK T. L.,
LINDSAY J. A.,
BUSTA F. F.
Publication year - 1987
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1987.tb01621.x
Subject(s) - escherichia coli , biology , dna , enterotoxin , hybridization probe , dna–dna hybridization , polymerase chain reaction , interchangeability , microbiology and biotechnology , gene , genetics , computer science , programming language
A colony hybridization method for visual detection of heat‐labile enterotoxin (LT)‐producing Escherichia coli has been developed. The interchangeability of probe DNA in the system offers the advantage of a more definitive indicator and detection procedure over current plating procedures. Preliminary studies have demonstrated complete specificity of the visual probe for target DNA. This model system has potential for use as a more definitive indicator method for the examination of foods and wastewater for LT toxin genes.