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Non‐toxic isolates of Bacillus thuringiensis producing parasporal inclusions with unusual protein components
Author(s) -
OHBA M.,
YU Y. M.,
AIZAWA K.
Publication year - 1987
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1987.tb01607.x
Subject(s) - bacillus thuringiensis , bacillaceae , bacillales , bombyx mori , biology , microbiology and biotechnology , pi , molecular mass , bacillus (shape) , bacteria , biochemistry , enzyme , bacillus subtilis , genetics , gene
Two strains of Bacillus thuringiensis isolated in Japan, designated 84‐F‐58‐20 and 84–F–58–35, produced unique parasporal inclusions (PI) non‐toxic to four insect species of three orders. Microscopic observation showed that the PI were rhombic but flat in shape. Intact PI of these isolates contained the major protein of 60 kD and several minor proteins. Upon dissolution of these PI with alkali (pH 10.2) con‐taining silkworm ( Bombyx mori ) larval gut juice, the 60 kD major protein was degraded and several proteins with lower molecular masses were detected in the supernatant fluid. Of these, the 27 kD protein was predominant.