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Serotyping of mesophilic Aeromonas spp. on the basis of lipopolysaccharide antigens
Author(s) -
FRICKER C. R.
Publication year - 1987
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1987.tb01595.x
Subject(s) - serotype , aeromonas , microbiology and biotechnology , lipopolysaccharide , antigen , biology , mesophile , virology , bacteria , immunology , genetics
A serotyping system has been developed for Aeromonas hydrophila, A. sobria and A. caviae based on lipopolysaccharide (LPS) antigens. Antigens are detected by slide agglutination of boiled cells and the serotype is confirmed by tube agglutination. The antigens involved in the serotyping reactions were shown to be capable of sensitizing chicken red blood cells and were extractable by ethyl‐enediaminetetraacetate. Furthermore, the reactions could be prevented by absorb‐ing antisera with purified LPS. Using 16 antisera, 63 of 137 (46%) strains isolated from human faeces could be serotyped.