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A method for the enrichment of auxotrophic mutants of Serratia marcescens with the antibiotic azthreonam
Author(s) -
TRIAS J.,
VIÑAS M.,
GUINEA J.,
LORÈN J. G.
Publication year - 1987
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1987.tb01588.x
Subject(s) - serratia marcescens , auxotrophy , mutant , microbiology and biotechnology , biology , serratia , enterobacteriaceae , bacteria , chemistry , escherichia coli , biochemistry , genetics , gene , pseudomonas
A new method for the enrichment of cultures of Serratia marcescens for auxotrophic mutants has been developed. The method is based on the formation of filaments by growing cells in minimal medium M70 containing azthreonam. Auxotrophic mutants unable to grow in M70 do not form filaments. Mutants are collected from the culture by filtration.