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pH‐dependent adsorption of cellulases to protein‐extracted lucerne fibres
Author(s) -
STUTZENBERGER F. J.
Publication year - 1987
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1987.tb01580.x
Subject(s) - cellulase , bioconversion , trichoderma reesei , adsorption , mesophile , hydrolysis , chemistry , thermophile , biochemistry , food science , microbiology and biotechnology , chromatography , enzyme , bacteria , biology , organic chemistry , fermentation , genetics
The capacity of protein‐extracted lucerne fibres to bind the cellulases of the mesophilic fungus Trichoderma reesei , and the thermophilic actinomycete Thermomonospora curvata , was determined to evaluate the fibre as a cellulase‐recycling vehicle during bioconversion processes. Adsorption of fungal and bacterial cellulase complexes was minimal at the pH optima (5‐0 and 6‐2 respectively) for fibre conversion to soluble sugars. Lowering of incubation temperature to 3°C enhanced adsorption of fungal cellulases, but had no effect on bacterial cellulase adsorption. However, adsorptive capacity for either could be improved about 30% by raising the pH above the hydrolysis optimum during the recycling phase.