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A simple method for detecting heparinase‐producing bacteria
Author(s) -
RILEY T. V.
Publication year - 1987
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1987.tb01573.x
Subject(s) - bacteria , simple (philosophy) , microbiology and biotechnology , biology , genetics , philosophy , epistemology
A simple method for detecting heparinase‐producing bacteria is described. The method is based on the metachromatic reaction between heparin and toluidine blue. Though developed primarily for Bacteroides spp., the method should find application in the detection of other aerobic and anaerobic heparinase‐producing bacteria, without the need for large amounts of media and expensive spectrophotometric equipment.