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Evaluation of three procedures using a laser densitometer and microcomputer for estimating molecular sizes of restriction endonuclease digest fragments and application to Campylobacter jejuni chromosomal DNA
Author(s) -
OWEN R. J.,
BECK A.
Publication year - 1987
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1987.tb01569.x
Subject(s) - restriction enzyme , densitometer , biology , dna , genetics , library science , computer science , physics , optics
Laser densitometer scanning and three methods of computation were investigated for estimating sizes of restriction endonuclease digest fragments of Campylobacter jejuni chromosomal DNA. Accuracy of the procedures was determined with standard bacteriophage lambda DNA fragments. The program DNASIZE was the most accurate for fragments between 2.3 and 33.5 kb while the program DNAFRAG and multiple regression analysis gave slightly greater errors particularly for smaller fragments. Hae III fingerprints of DNA from six C. jejuni strains contained at least 25 resolvable fragments with sizes between 3.2 and 35.8 kb estimated by DNASIZE and several differences between pattern types were detected.