Premium
Storage and survival of bacteria by ultra‐freeze
Author(s) -
GIBSON L. F.,
KHOURY J. T.
Publication year - 1986
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1986.tb01565.x
Subject(s) - subculture (biology) , bacteria , neisseria gonorrhoeae , biology , microbiology and biotechnology , strain (injury) , genetics , anatomy
The storage and survival of 97 species (276 strains) of bacteria at – 70° to – 80°C was investigated. Each strain was subcultured at regular intervals over a period of 6–40 months in order to check its viability. All strains were viable after the latest subculture except strains of Neisseria gonorrhoeae which proved non‐viable when tested after 12 months. The method proved most useful for storing stock cultures which required frequent and regular accessing.