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Acid resistance of attached organisms and its implications for the pathogenicity of plasmid‐bearing Escherichia coli
Author(s) -
POYNTER DIANE,
HICKS S. J.,
ROWBURY R. J.
Publication year - 1986
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1986.tb01563.x
Subject(s) - escherichia coli , microbiology and biotechnology , enterobacteriaceae , phagocyte , plasmid , biology , bacteria , chemistry , phagocytosis , biochemistry , dna , genetics , gene
Organisms of Escherichia coli attached to glass beads in a model attachment system were more resistant to acid than were unattached organisms and this applied to cultures exposed to either pH 2·5 or 3·5. Attached organisms of both Col ‐ and Col V, I‐K94 + strains showed the effect but with prolonged exposure to pH 2·5, the attached Col V + organisms appeared more resistant than the attached Col ‐ ones, possibly because they formed a thicker surface layer. It is proposed that the increased resistance of attached organisms to pH 2·5 might allow the survival, in gastric acid, of organisms attached to food particles. This would be more significant for the Col V + strains because the plasmid enhances enterobacterial attachment properties. The increased resistance of attached organisms to pH 3·5 might be significant for survival of particle‐ or surface‐bound organisms in the acidic environment of the phagocyte especially since lactate (which occurs in phagocytes) enhanced the effect of exposure to pH 3·5.

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