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A red/white selection for Saccharomyces cerevisiae auxotrophs
Author(s) -
PEARSON B. M.,
FULLER LINDA J.,
MACKENZIE D. A.,
KEENAN M. H. J.
Publication year - 1986
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1986.tb01555.x
Subject(s) - library science , clinical microbiology , auxotrophy , microbiology and biotechnology , medicine , biology , computer science , genetics , escherichia coli , gene
Red pigment production in yeast cells with adel or ade2 mutations has been exploited to develop a method of identifying specific amino acid auxotrophs. Amino acid auxotrophs carrying mutations in adel or ade2 show delayed pigment production at sub‐optimal amino acid levels. This delay allows selection of amino acid auxotrophs following mutagenesis, since red pigment is produced in prototrophs whilst auxotrophs remain white. This differential colour reaction has been applied to select leucine, lysine and serine auxotrophs. Large numbers of colonies could easily be screened without the need for extensive replica plating.