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A monoclonal antibody to ochratoxin A
Author(s) -
CANDLISH A. A. G.,
STIMSON W. H.,
SMITH J. E.
Publication year - 1986
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1986.tb01535.x
Subject(s) - library science , medicine , computer science
A monoclonal antibody (McAb) specific for ochratoxin A (OA) has been developed. The McAb has low cross‐reactivity for ochratoxin α, although cross‐reactivity with the synthetic ochratoxin C was high. The affinity dissociation constant of the McAb was found to be 6‐2 × 10 7 M ‐1 by ELISA. A competitive enzyme immunoassay was developed with this McAb having a sensitivity of 10 μg/ml and a working range up to 10 μ/ml of OA in standard solutions.