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Cryopreservation of the anaerobic rumen fungus Neocallimastix patriciarum
Author(s) -
YARLETT N. C.,
YARLETT N.,
ORPIN C. G.,
LLOYD D.
Publication year - 1986
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1986.tb01533.x
Subject(s) - cryoprotectant , cryopreservation , rumen , biology , tray , fungus , food science , microbiology and biotechnology , chemistry , botany , embryo , fermentation
Neocallimastix patriciarum was successfully frozen in liquid media containing 5% dimethylsuphoxide as cryoprotectant. Initial freezing and storage for 24 h in solid CO 2 before transfer to liquid N 2 was necessary for survival. Long‐term storage under liquid N 2 , solid CO 2 and at ‐ 80°C was tested; only storage in liquid N 2 proved successful for periods in excess of several months. Analysis of metabolic end‐products and electron microscopy indicated that no morphological or biochemical changes had occurred in cultures preserved for 1 year. Piromonas communis and a recent isolate from the ovine rumen were preserved under the same conditions. The method does not require controlled freezing equipment, making it an easily applied laboratory technique.