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Detection of mycobacterial lipid antigens by a combination of thin‐layer chromatography and immunostaining
Author(s) -
Ridell Malin,
Minnikin D. E.,
Parlett J. H.,
MattsbyBaltzer Inger
Publication year - 1986
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1986.tb01522.x
Subject(s) - antiserum , immunostaining , antigen , strain (injury) , glycolipid , serotype , thin layer chromatography , biology , phosphatidylinositol , chemistry , microbiology and biotechnology , biochemistry , chromatography , immunology , immunohistochemistry , anatomy , kinase
An immunostaining method is described for the direct detection of mycobacterial lipid antigens on thin‐layer chromatograms, no prior purification of the crude lipid extract is required. Analysis of Mycobacterium scrofulaceum serovar 41 gave positive reactions for the polar, but not the non‐polar, glycopeptidolipids, with an antiserum against whole cells of the same strain. The family of phosphatidylinositol mannosides did not react with this antiserum but a reaction was obtained with an antiserum against intracellular material from another strain.