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Some properties of cefoxitin‐induced spheroplasts of Proteus species, including sensitivity to colistin
Author(s) -
Chapman D. G.,
Russell A. D.
Publication year - 1986
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1986.tb01520.x
Subject(s) - welsh , colistin , cefoxitin , library science , spheroplast , medicine , microbiology and biotechnology , biology , geography , archaeology , computer science , antibiotics , bacteria , escherichia coli , biochemistry , genetics , staphylococcus aureus , gene
Spheroplasts of various Proteus species could be induced by cefoxitin in nutrient broth alone or in broth containing 0–33 mol/l sucrose and 4 times 10 ‐ 3 mol/l magnesium sulphate. Spheroplasts induced in the latter medium were lysed when diluted into water, but osmotically stable if transferred to broth or other appropriate menstrua. High concentrations of colistin were needed to induce lysis of cefoxitin‐induced spheroplasts and it was concluded that, contrary to earlier findings with benzylpenicillin‐induced spheroplasts, peptidoglycan had little, if any, role to play in acting as a barrier to the entry of polymyxins into Proteus cells.