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The preservation of lactobacilli: A comparison of three methods
Author(s) -
Barbour E. Anne,
Priest F. G.
Publication year - 1986
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1986.tb01518.x
Subject(s) - library science , clinical microbiology , history , biology , computer science , microbiology and biotechnology
Lyophilization, desiccation on pumice stone and storage in dilute glycerol at‐20d̀C were compared for the preservation of Lactobacillus cultures. After storage for two years, 73% of the lyophilized cultures were viable. Desiccation on pumice stone gave erratic results and is not recommended. Storage in 20% (v/v) glycerol at‐20d̀C is simple and convenient and after 24 months, 75% of the cultures were viable. It is a useful method of maintaining routinely accessed cultures.