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A note on the use of a scanning laser densitometer to analyse silver‐stained lipopolysaccharide patterns on polyacrylamide gels and antigenic profiles on nitrocellulose sheets produced by immunoblotting
Author(s) -
Tilling R. K.,
Jessop Helen L.,
Lambert P. A.
Publication year - 1986
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1986.tb01512.x
Subject(s) - densitometer , nitrocellulose , art history , library science , microbiology and biotechnology , biology , polymer science , chemistry , optics , art , physics , computer science , biochemistry , membrane
Silver‐stained patterns of lipopolysaccharide on sodium dodecyl sulphate polyacrylamide gels and microbial antigenic profiles on nitrocellulose sheets produced by electroblotting and immunodetection were analysed with a laser scanning densitometer. Because the patterns fade rapidly it is usual to photograph them immediately to obtain optimum resolution. It has been shown that because of its high resolution the laser densitometer can be used to scan 35 mm photographic negatives without appreciable loss of detail. The method might be of value with whole cell immunoblot antigen profiles as characteristic fingerprints, for example, in taxonomic studies.