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Separation of autoinhibitor from cytokinin activity in spores of Dictyostelium discoideum
Author(s) -
Leighton Fiona C.,
Hutchinson J. S.M.,
Seddon B.
Publication year - 1985
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1985.tb01501.x
Subject(s) - dictyostelium discoideum , cytokinin , spore , sephadex , zeatin , biochemistry , biology , mycetozoa , slime mold , chemistry , botany , enzyme , auxin , gene
Spores of Dictyostelium discoideum NC‐4H contain a spore germination autoinhibitor (SGI) that, on purification using Sephadex LH‐20 eluted with 35% ethanol, can be separated from the bulk of cytokinin activity. Inhibitor studies and chromatographic analysis indicate that SGI is not a known cytokinin such as zeatin or IPA (N 6 ‐(δ 2 ‐isopentenyl)‐adenine), N 2 ‐dimethylguanosine or discadenine. Considering these findings and conflicting views in the literature concerning the structure of the autoinhibitor, the identity of SGI is discussed.