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Formation and regeneration of protoplasts of Streptomyces hygroscopicus
Author(s) -
Hooley P.,
Wellington A. M. H.
Publication year - 1985
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1985.tb01494.x
Subject(s) - protoplast , streptomyces hygroscopicus , regeneration (biology) , biology , mycelium , streptomyces , botany , streptomycetaceae , microbiology and biotechnology , bacteria , actinomycetales , genetics
Techniques are described for the production and regeneration of protoplasts of Streptomyces hygroscopicus and related strains. No single technique was successful in producing protoplasts from all strains. Regeneration of protoplasts to the mycelial growth form was greatly improved by modifying the physical regeneration environment. Protoplast formation and regeneration was achieved in six out of the seven strains studied.