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A monoclonal antibody to aflatoxin B 1 : detection of the mycotoxin by enzyme immunoassay
Author(s) -
Candlish A. A.G.,
Stimson W. H.,
Smith J. E.
Publication year - 1985
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1985.tb01489.x
Subject(s) - library science , george (robot) , mycotoxin , aflatoxin , immunoassay , medicine , microbiology and biotechnology , biology , art , art history , antibody , computer science , immunology
A monoclonal antibody (McAb) was produced after fusion of mouse (X63.Ag8.6.5.3) myeloma cells with spleen cells isolated from female Balb‐c/NZB F1 hybrid mice immunized with aflatoxin B 1 (oxine)‐keyhole limpet haemocyanin conjugate. The hybridoma cell line producing antibody specific for aflatoxin B 1 (AFB 1 ) was grown in tissue culture and as an ascites tumour. The ascitic fluid gave suitably high dilution titres (1:800 000) by enzyme immunoassay and was conjugated to horseradish peroxidase by a two‐step procedure with glutaraldehyde. The conjugate was used to develop a direct competitive enzyme‐linked immunosorbent (ELISA) assay for AFB 1 . The sensitivity of the ELISA was 0–2 ng/ml with a working range up to 10 ng/ml for AFB 1 . The specificity of the McAb was determined and it was shown not to cross‐react significantly with any of the metabolites tested. This McAb and the direct competitive ELISA described may prove of use in the detection of AFB 1 in foods and feeds.