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Effect of chlorhexidine on a chlorhexidine‐sensitive and a chlorhexidine‐resistant strain of Providencia stuartii
Author(s) -
ElMoug T.,
Furr J. R.,
Russell A. D.
Publication year - 1985
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.1985.tb01484.x
Subject(s) - chlorhexidine , microbiology and biotechnology , strain (injury) , chemistry , biology , medicine , dentistry , anatomy
The sensitivity and resistance of three strains of Providencia stuartii to various antibacterial agents, and especially to chlorhexidine, are described. Providencia stuartii Pv 2 was the most sensitive, and Pv 67 the most resistant, to chlorhexidine and to polymyxin B. These two strains took up approximately equal amounts of chlorhexidine from solution, but the biguanide had a considerably greater effect on the electrophoretic mobility of cells of strain Pv 2. Greater inner membrane damage (determined by the leakage of K + and of pentoses) occurred with Pv 2. Chlorhexidine at 20 μg/ml achieved a 2‐log reduction and 50 μg/ml a > 7‐log reduction in viable numbers in strain Pv 2 over a 120 min contact period at 20d̀C. In contrast, these concentrations induced < 0.5 log reduction in strain Pv 67.