Premium
Isolation and characterization of 11 polymorphic microsatellite loci in the whitegirdled goby ( Pterogobius zonoleucus ) and cross‐species amplification in the serpentine goby ( P. elapoides )
Author(s) -
NOHARA K.,
KOKITA T.,
TOMINAGA O.,
SEIKAI T.
Publication year - 2009
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2009.00228_9_2.x
Subject(s) - biology , microsatellite , linkage disequilibrium , locus (genetics) , goby , genetics , allele , population , haplotype , gene , fishery , fish <actinopterygii> , demography , sociology
Eleven polymorphic microsatellite markers were developed from a (CA) n ‐enrichment library of the whitegirdled goby ( Pterogobius zonoleucus ). Polymorphism at these loci ranged from 2 to 12 alleles, and observed and expected heterozygosities from 0.05 to 0.90 and from 0.05 and 0.86, respectively. All loci conformed to Hardy–Weinberg equilibrium, with no significant linkage disequilibrium between all locus pairs. Cross‐species amplification tests were successful in P. elapoides , and most loci were polymorphic. These microsatellite markers will be useful in further population genetic studies of both species.