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Characterization of microsatellite loci in the lesser dawn bat ( Eonycteris spelaea )
Author(s) -
SHAO W. W.,
HUA P. Y.,
ZHOU S. Y.,
ZHANG S. Y.,
CHEN J. P.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.02051.x
Subject(s) - microsatellite , biology , linkage disequilibrium , locus (genetics) , genetics , loss of heterozygosity , population , allele , evolutionary biology , haplotype , gene , demography , sociology
We constructed a genomic DNA library enriched for CA repeat motifs in Eonycteris spelaea . Nine microsatellite loci were isolated and tested on a population of 39 samples from Yunnan Province, China. These nine loci had three to 22 alleles per locus. Observed and expected heterozygosity values ranged from 0.079 to 0.963 and from 0.078 to 0.959. Two loci revealed significant departure from Hardy–Weinberg equilibrium and no linkage disequilibrium was found between loci pairs. These microsatellites can be a powerful molecular tool for population‐level studies of E. spelaea .