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Isolation and characterization of 10 tetranucleotide microsatellite loci from the yellow‐streaked greenbul ( Phyllastrephus flavostriatus ) and cross‐species amplification in four closely related taxa
Author(s) -
LOKUGALAPPATTI L. G. SAMPATH,
FELDHEIM KEVIN A.,
SELLAS ANNA B.,
BOWIE RAURI C. K.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.02023.x
Subject(s) - biology , microsatellite , loss of heterozygosity , linkage disequilibrium , genetics , allele , evolutionary biology , taxon , haplotype , gene , botany
We describe the isolation of 10 tetranucleotide microsatellite loci from the yellow‐streaked greenbul using an enrichment protocol. All loci were highly variable with the number of alleles ranging from 8 to 13, and observed heterozygosity ranging from 0.652 to 0.870. All loci were in Hardy–Weinberg equilibrium; however, loci Pfl12 and Pfl54 showed significant linkage disequilibrium. All 10 loci successfully amplified and were polymorphic in at least one of four related Phyllastrephus species. These loci should prove to be widely applicable to studies of phylogeography, hybridization and paternity in African greenbuls.