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Isolation and characterization of microsatellite markers in black muntjac ( Muntiacus crinifrons )
Author(s) -
WU HAILONG,
MENG KAI,
ZHU GUOPING
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.02003.x
Subject(s) - biology , microsatellite , isolation (microbiology) , evolutionary biology , genetics , bioinformatics , gene , allele
The black muntjac ( Muntiacus crinifrons ) is a vulnerable species found in the mountains of eastern China, about which little is known. Here we develop 11 polymorphic microsatellite loci from a (CA) n ‐enrichment library for the animal. In the analyses of 25 individuals sampled, unbiased expected heterozygosity levels varied from 0.686 to 0.876 and the number of alleles per locus varied from five to nine. Results that 11 microsatellite loci were highly polymorphic indicated that these markers are sufficiently powerful to address such questions as parentage, mating system and population genetic structure of M. crinifrons .