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Isolation and characterization of 12 dinucleotide microsatellite loci in the anadromous fish Osmerus eperlanus (L.)
Author(s) -
TAYLOR A. N.,
TAYLOR M. I.,
MCCARTHY I. D.,
BEAUMONT A. R.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.02001.x
Subject(s) - biology , microsatellite , loss of heterozygosity , locus (genetics) , fish migration , genetics , population , smelt , allele , population genetics , fish <actinopterygii> , gene , fishery , demography , sociology
Abstract A subtractive hybridization method was used to isolate 12 dinucleotide microsatellite loci for the anadromous European smelt, Osmerus eperlanus (L.). Three to 17 (mean 8.08) alleles per locus were identified in the two populations screened, and observed heterozygosity ranged from 0.067 to 0.933. Loci in both populations showed significant deviations from Hardy–Weinberg expected frequencies. These 12 loci provide a good basis for investigation of O. eperlanus population structure.