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PERMANENT GENETIC RESOURCES: Consensus primers of cyp73 genes discriminate willow species and hybrids ( Salix , Salicaceae)
Author(s) -
TRUNG LE QUANG,
VAN PUYVELDE KAROLIEN,
TRIEST LUDWIG
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01991.x
Subject(s) - biology , salicaceae , hybrid , willow , genetics , gene , allele , intron , botany , woody plant
Consensus primers, based on exon sequences of the cyp73 gene family coding for cinnamate 4‐hydroxylase (C4H) of the lignin biosynthesis pathway, were designed for the tetraploid willow species Salix alba and Salix fragilis . Diagnostic alleles at species level were observed among introns of three cyp73 genes and allowed unambiguous detection of the first generation and introgressed hybrids in populations. Progeny analysis of a female S. alba with a male introgressed hybrid confirmed the codominant inheritance of each intron. Sequences of the diagnostic alleles of both species were similar to those found in the hybrids.