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PERMANENT GENETIC RESOURCES: Isolation and characterization of microsatellite loci in the cichlid fish in Lake Victoria, Haplochromis chilotes
Author(s) -
MAEDA K.,
TAKESHIMA H.,
MIZOIRI S.,
OKADA N.,
NISHIDA M.,
TACHIDA H.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01981.x
Subject(s) - cichlid , biology , microsatellite , loss of heterozygosity , zoology , evolutionary biology , characidae , allele , genetics , fish <actinopterygii> , fishery , gene
Twelve short tandem repeat markers were successfully isolated from a cichlid, Haplochromis chilotes , in Lake Victoria, and characterized in Haplochromis pyrrhocephalus . The microsatellite regions of these markers were found to have between two and 48 alleles with heterozygosity ranging from 0.07 to 0.97. No loci showed significant departures from the Hardy–Weinberg or linkage equilibrium after the Bonferroni correction ( P > 0.05). Cross‐species amplification in other cichlids of Lake Victoria, Haplochromis laparogramma , Lithochromis rubripinnis , L. rufus and Haplochromis sp. ‘rockkribensis’, was successful.