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PERMANENT GENETIC RESOURCES: Microsatellite markers for the threatened Bliss Rapids snail ( Taylorconcha serpenticola ) and cross‐amplification in its congener, T. insperata
Author(s) -
LIU H. P.,
HERSHLER R.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01977.x
Subject(s) - biology , threatened species , congener , microsatellite , bliss , zoology , genetics , evolutionary biology , ecology , allele , habitat , gene , chemistry , chromatography , computer science , programming language
Abstract We developed and tested microsatellite markers to investigate population structure of a threatened North American freshwater gastropod, Taylorconcha serpenticola . Of the 21 primer pairs that were evaluated, 11 were readily optimized and scored, providing amplification of 12 loci that were screened for 820 specimens from 29 populations. The number of alleles across 11 of these polymorphic loci ranged from three to 20 and the observed heterozygosity varied from 0.0061 to 0.7561. All loci yielded suitable amplification products in the second species of Taylorconcha ( T. insperata ) and three proved to be diagnostic for these congeners, demonstrating that these markers are also useful for species identification studies.