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PERMANENT GENETIC RESOURCES: Isolation and characterization of 12 microsatellite markers in the middle‐spotted woodpecker ( Dendrocopos medius )
Author(s) -
VILA M.,
ROBLES H.,
CIUDAD C.,
OLEA P. P.,
BAGLIONE V.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01976.x
Subject(s) - biology , microsatellite , woodpecker , linkage disequilibrium , locus (genetics) , genetic diversity , population , loss of heterozygosity , genetic structure , allele , medius , zoology , ecology , evolutionary biology , genetics , genetic variation , habitat , haplotype , demography , anatomy , sociology , gene
Twelve polymorphic microsatellite loci were developed for Dendrocopos medius. Polymorphism was assessed for 27 individuals from the southwesternmost population of this woodpecker species. The number of alleles per locus ranged from three to seven, with observed heterozygosity values from 0.444 to 0.852. Genotypic frequencies conformed to Hardy–Weinberg equilibrium, and no evidence for linkage disequilibrium was observed. Multilocus genotypes resulting from this set of markers will be useful to determine genetic diversity and differentiation within and among habitat patches inhabited by D. medius . Three of the loci were polymorphic for Picoides articus .