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PERMANENT GENETIC RESOURCES: PCR primers for 100 microsatellites in red drum ( Sciaenops ocellatus )
Author(s) -
KARLSSON STEN,
RENSHAW MARK A.,
REXROAD III CAIRD E.,
GOLD JOHN R.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01969.x
Subject(s) - microsatellite , biology , allele , genetics , genetic diversity , null allele , population , genotype , evolutionary biology , gene , demography , sociology
One hundred nuclear‐encoded microsatellites from a genomic library of red drum ( Sciaenops ocellatus ) were isolated and characterized. Eight microsatellites had tetranucleotide motifs; 92 had dinucleotide motifs. The average number of alleles per microsatellite (sample of 22–24 fish) was 17.7 (range = 2–30); gene diversity averaged 0.796 (range = 0.227–1.000). Following Bonferroni correction, genotype frequencies at 90 microsatellites did not deviate significantly from Hardy–Weinberg equilibrium expectations. Occurrence of null alleles was inferred at 15 microsatellites; alleles differing by only a single base were observed at 11 microsatellites. The microsatellites developed should prove useful for population‐genetic studies of ‘wild’ red drum and in construction of a genetic map.