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PERMANENT GENETIC RESOURCES: Isolation of 60 polymorphic microsatellite loci in EST libraries of four sibling species of the phytopathogenic fungal complex Microbotryum
Author(s) -
GIRAUD T.,
YOCKTENG R.,
MARTHEY S.,
CHIAPELLO H.,
JONOT O.,
LOPEZVILLAVICENCIO M.,
DE VIENNE D. M.,
HOOD M. E.,
REFREGIER G.,
GENDRAULTJACQUEMARD A.,
WINCKER P.,
DOSSAT C.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01967.x
Subject(s) - biology , microsatellite , silene , genetics , botany , gene , allele
We report the development of 60 microsatellite markers on four species of the fungal complex Microbotryum , causing anther smut of the Caryophyllaceae. Microsatellites were found in four expressed sequence tag (EST) libraries, built from isolates of M. lychnis‐dioicae, M. violaceum sensus stricto, M. lagerheimii and M. dianthorum , collected, respectively, from the plants Silene latifolia, S. nutans, S. vulgaris and Dianthus carthusianorum . Intrapopulation polymorphism was investigated using 24 isolates, and cross‐amplification was explored using 23 isolates belonging to at least 10 different Microbotryum species. This study provides numerous microsatellite markers for population genetics and mapping studies.