Premium
PERMANENT GENETIC RESOURCES: Isolation and characterization of microsatellite DNA loci from the southern flounder, Paralichthys lethostigma
Author(s) -
SHAO C. W.,
LIAO X.,
CHEN S. L.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01965.x
Subject(s) - biology , microsatellite , genetics , locus (genetics) , allele , paralichthys , genetic diversity , population , zoology , evolutionary biology , fishery , fish <actinopterygii> , gene , demography , sociology
Paucity of polymorphic molecular markers in southern flounder ( Paralichthys lethostigma ) has been a major limitation in genetic improvement of this important economic fish. Hence, we constructed a repeat‐enriched genomic library from P. lethostigma. A total of 39 new microsatellites were identified, for which 33 primer pairs were designed. After validating and scoring, 10 of these loci were polymorphic in a test population with the range of alleles from two to nine per locus. The observed and expected heterozygosities ranged from 0.2500 to 0.9000 and from 0.4469 to 0.8514, respectively. These polymorphic microsatellites will be useful for genetic diversity analysis and linkage map construction for P. lethostigma.