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PERMANENT GENETIC RESOURCES: Polymorphic microsatellite loci for Diachasma alloeum (Hymenoptera: Braconidae)
Author(s) -
FORBES ANDREW A.,
POWELL THOMAS H. Q.,
LOBO NEIL F.,
NOOR MOHAMED A. F.,
FEDER JEFFREY L.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01961.x
Subject(s) - braconidae , biology , rhagoletis , microsatellite , sympatric speciation , hymenoptera , parasitoid , zoology , evolutionary biology , tephritidae , pest analysis , genetics , botany , allele , gene
Abstract Here, we report the isolation of 21 novel primers for amplification of microsatellite loci in Diachasma alloeum (Hymenoptera: Braconidae). Diachasma alloeum is a larval parasitoid of the apple maggot fly ( Rhagoletis pomonella ), which is an economically significant agricultural pest species and a textbook example of sympatric speciation via host‐plant shifting. These microsatellite markers will prove useful both for assessing genetic relationships between different host‐associated populations of D. alloeum , as well as for future R. pomonella biological control programmes. We also report the cross‐species amplification of several loci for Diachasmimorpha mellea and Diachasma ferrugineum, parasitoids of R. pomonella and R. cingulata , respectively.