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PERMANENT GENETIC RESOURCES: Isolation of microsatellite loci from the endangered plant Sibara filifolia (Brassicaceae)
Author(s) -
MCGLAUGHLIN MITCHELL E.,
WALLACE LISA E.,
HELENURM KAIUS
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01958.x
Subject(s) - biology , brassicaceae , microsatellite , linkage disequilibrium , endangered species , genetic variation , botany , genetic variability , allele , zoology , evolutionary biology , genetics , ecology , haplotype , genotype , gene , habitat
Sibara filifolia (Brassicaceae) is a federally endangered annual herb found on two of the California Channel Islands. Previous studies based on allozymes revealed little genetic variability on San Clemente Island. Nine polymorphic microsatellite loci were isolated from individuals on San Clemente Island. We found low levels of allelic variation (mean N A = 2.3), with seven loci exhibiting significant deviations from Hardy–Weinberg equilibrium ( P < 0.01) and 10 pairs of loci exhibiting significant linkage disequilibrium ( P < 0.01). Most of the observed variability (mean H O = 0.003) occurred among populations or in rare homozygous individuals.