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PERMANENT GENETIC RESOURCES: A set of 12 microsatellite loci for genetic studies of Leishmania braziliensis
Author(s) -
ROUGERON V.,
WALECKX E.,
HIDE M.,
DE MEEÛS T.,
AREVALO J.,
LLANOSCUENTAS A.,
BAÑULS A. L.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01953.x
Subject(s) - biology , loss of heterozygosity , microsatellite , leishmania braziliensis , genetics , allele , heterozygote advantage , population , population genetics , leishmania , evolutionary biology , leishmaniasis , cutaneous leishmaniasis , gene , parasite hosting , demography , sociology , world wide web , computer science
Twelve microsatellite loci of Leishmania braziliensis were examined, nine of which were developed in this work. Fifty‐six Leishmania braziliensis were genotyped with these microsatellite loci. The 12 loci studied were polymorphic with the number of alleles ranging from five to 19, with a mean of 9.7 ± 4.1 and the observed heterozygosity averaging 0.425 ± 0.202. The important heterozygote deficits we observed ( F IS = 0.41, P value = 0.004) appear incompatible with the heterozygote excess expected in clonal diploids. This last result could revive the clonality/sexuality debate regarding Leishmania . This work validates the potential use of these microsatellites for population genetics analysis.