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PERMANENT GENETIC RESOURCES: Development of microsatellite markers for the guava rust fungus, Puccinia psidii
Author(s) -
ZHONG SHAOBIN,
YANG BAOJUN,
ALFENAS ACELINO COUTO
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01952.x
Subject(s) - biology , microsatellite , loss of heterozygosity , genetics , rust (programming language) , population , primer (cosmetics) , fungus , genomic dna , genetic marker , allele , botany , dna , gene , chemistry , demography , organic chemistry , sociology , computer science , programming language
We developed and characterized 15 polymorphic microsatellite markers present in the genome of the guava rust fungus, Puccinia psidii . The primers for these microsatellite markers were designed by sequencing clones from a genomic DNA library enriched for a simple sequence repeat (SSR) motif of (AG). All these 15 primer pairs successfully amplified DNA fragments from a sample of 22 P. psidii isolates, revealing a total of 71 alleles. The observed heterozygosity at the 15 loci ranged from 0.05 to 1.00. The SSR markers developed would be useful for population genetics study of the rust fungus.