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PERMANENT GENETIC RESOURCES: Eighteen new polymorphic microsatellite markers for the endangered Florida manatee, Trichechus manatus latirostris
Author(s) -
TRINGALI MICHAEL D.,
SEYOUM SEIFU,
CARNEY SUSAN L.,
DAVIS MICHELLE C.,
RODRIGUEZLOPEZ MARTA A.,
REYNOLDS III JOHN E.,
HAUBOLD ELSA
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01944.x
Subject(s) - biology , microsatellite , manatee , locus (genetics) , zoology , endangered species , linkage disequilibrium , genotype , allele , genetics , ecology , habitat , haplotype , gene
Here we describe 18 polymorphic microsatellite loci for Trichechus manatus latirostris (Florida manatee), isolated using a polymerase chain reaction‐based technique. The number of alleles at each locus ranged from two to four (mean = 2.5) in specimens from southwest ( n = 58) and northeast ( n = 58) Florida. Expected and observed heterozygosities ranged from 0.11 to 0.67 (mean = 0.35) and from 0.02 to 0.78 (mean = 0.34), respectively. Departures from Hardy–Weinberg equilibrium occurred at two loci. There was no evidence of genotypic disequilibrium for any pair of loci. For individual identification, mean random‐mating and θ‐corrected match probabilities were 9.36 × 10 −7 and 1.95 × 10 −6 , respectively.