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Thirteen polymorphic microsatellite DNA loci from whiptails of the genus Aspidoscelis (Teiidae: Squamata) and related cnemidophorine lizards
Author(s) -
CRAWFORD NICHOLAS G.,
ZALDÍVARRAE JAIME,
HAGEN CRIS,
SCHABLE AMANDA,
ROSENBLUM ERICA BREE,
GRAVES JEFF A.,
REEDER TOD W.,
RITCHIE MICHAEL G.,
GLENN TRAVIS C.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01930.x
Subject(s) - biology , teiidae , microsatellite , locus (genetics) , squamata , loss of heterozygosity , allele , genetics , lizard , zoology , polymerase chain reaction , gene , sauria
We describe polymerase chain reaction primers and amplification conditions for 13 microsatellite DNA loci isolated from two bisexual species of whiptail lizards Aspidoscelis costata huico and Aspidoscelis inornata . Primers were tested on either 16 or 48 individuals of A. c. huico and/or 26 individuals of A. inornata. Ten of the 13 primers were also tested against a panel of 31 additional whiptail taxa. We detected three to nine alleles per locus in A. c. huico and four to 19 alleles per locus in A. inornata , with observed heterozygosity ranging from 0.60 to 0.87 and from 0.15 to 1.00, respectively. These primers will be an important resource for surveys of genetic variation in these lizards.