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Isolation and characterization of polymorphic microsatellite loci from RAPD product in half‐smooth tongue sole ( Cynoglossus semilaevis ) and a test of cross‐species amplification
Author(s) -
LIU Y. G.,
BAO B. L.,
LIU L. X.,
WANG L.,
LIN H.
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01923.x
Subject(s) - biology , microsatellite , genetics , rapd , primer (cosmetics) , allele , gene , population , genetic diversity , chemistry , demography , organic chemistry , sociology
Eleven polymorphic microsatellite loci have been isolated and characterized from random amplified polymorphic DNA product in half‐smooth tongue sole, Cynoglossus semilaevis . Twenty‐one microsatellites were selected for designing microsatellite primers, of which 11 gave working primer pairs. They had between three and 12 alleles. Observed and expected heterozygosities varied from 0.53 to 0.93, and from 0.52 to 0.80, respectively. Five additional fish species assessed for cross‐species amplification revealed between one and three positive amplifications and between zero and three polymorphic loci per species.