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Characterization of microsatellite loci for the endangered cactus Echinocactus grusonii , and their cross‐species utilization
Author(s) -
HARDESTY B. D.,
HUGHES S. L.,
RODRIGUEZ V. M.,
HAWKINS J. A
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01913.x
Subject(s) - biology , microsatellite , endangered species , cactus , primer (cosmetics) , critically endangered , population , habitat , zoology , evolutionary biology , genetics , ecology , allele , gene , chemistry , demography , organic chemistry , sociology
Echinocactus grusonii is common in trade but critically endangered in its natural habitat. With the ultimate aim of developing a certification scheme to aid in the conservation of this species, we have isolated E. grusonii microsatellites from a nonenriched library. Fifty‐seven sequences contained a microsatellite array, of which 12 were polymorphic among 30 individuals from a single wild population. All 12 microsatellite primer pairs amplified product in one or more species in a screen of 27 other cactus species.