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Isolation, characterization and multiplex polymerase chain reaction of novel microsatellite loci for the avian parasite Philornis downsi (Diptera: Muscidae)
Author(s) -
DUDANIEC RACHAEL Y.,
GARDNER MICHAEL G.,
KLEINDORFER SONIA
Publication year - 2008
Publication title -
molecular ecology resources
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.96
H-Index - 136
eISSN - 1755-0998
pISSN - 1755-098X
DOI - 10.1111/j.1471-8286.2007.01900.x
Subject(s) - biology , muscidae , microsatellite , parasite hosting , polymerase chain reaction , loss of heterozygosity , multiplex polymerase chain reaction , genetics , population , zoology , allele , gene , demography , sociology , world wide web , computer science
An enrichment technique was used to isolate 11 di‐, tri‐, and tetra microsatellites for the parasitic fly Philornis downsi (Diptera: Muscidae). These loci were polymerase chain reaction amplified in singleplexes or two‐plexes for P. downsi . The loci showed low to moderate polymorphism, exhibited between three and four alleles, and observed heterozygosity ranged from 0.05 to 0.86. These new markers will be useful for population‐level and paternity analyses and will provide valuable information about the ecology of this high‐impact parasite of vulnerable bird species.