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Vesicular uptake blockade generates the toxic dopamine metabolite 3,4‐dihydroxyphenylacetaldehyde in PC 12 cells: relevance to the pathogenesis of Parkinson's disease
Author(s) -
Goldstein David S.,
Sullivan Patti,
Cooney Adele,
Jinsmaa Yunden,
Sullivan Rachel,
Gross Daniel J.,
Holmes Courtney,
Kopin Irwin J.,
Sharabi Yehonatan
Publication year - 2012
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2012.07924.x
Subject(s) - dopamine , vesicular monoamine transporter , dopaminergic , reserpine , biochemistry , monoamine oxidase , biology , aromatic l amino acid decarboxylase , monoamine neurotransmitter , chemistry , pharmacology , endocrinology , medicine , enzyme , serotonin , receptor
Parkinson's disease entails profound loss of nigrostriatal dopaminergic terminals, decreased vesicular uptake of intraneuronal catecholamines, and relatively increased putamen tissue concentrations of the toxic dopamine metabolite, 3,4‐dihydroxyphenylacetaldehyde ( DOPAL ). The objective of this study was to test whether vesicular uptake blockade augments endogenous DOPAL production. We also examined whether intracellular DOPAL contributes to apoptosis and, as α‐synuclein oligomers may be pathogenetic in Parkinson's disease, oligomerizes α‐synuclein. Catechols were assayed in PC 12 cells after reserpine to block vesicular uptake, with or without inhibition of enzymes metabolizing DOPAL —daidzein for aldehyde dehydrogenase and AL1576 for aldehyde reductase. Vesicular uptake was quantified by a method based on 6F‐ or 13 C‐dopamine incubation; DOPAL toxicity by apoptosis responses to exogenous dopamine, with or without daidzein+AL1576; and DOPAL ‐induced synuclein oligomerization by synuclein dimer production during DOPA incubation, with or without inhibition of L‐aromatic‐amino‐acid decarboxylase or monoamine oxidase. Reserpine inhibited vesicular uptake by 95–97% and rapidly increased cell DOPAL content ( p = 0.0008). Daidzein+AL1576 augmented DOPAL responses to reserpine ( p = 0.004). Intracellular DOPAL contributed to dopamine‐evoked apoptosis and DOPA ‐evoked synuclein dimerization. The findings fit with the ‘catecholaldehyde hypothesis,’ according to which decreased vesicular sequestration of cytosolic catecholamines and impaired catecholaldehyde detoxification contribute to the catecholaminergic denervation that characterizes Parkinson's disease.