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Evidence for a systemic regulation of neurotrophin synthesis in response to peripheral nerve injury
Author(s) -
Shakhbazau Antos,
Martinez Jose A.,
Xu QingGui,
Kawasoe Jean,
van Minnen Jan,
Midha Rajiv
Publication year - 2012
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2012.07792.x
Subject(s) - nerve growth factor , neurotrophin , neurotrophin 3 , sciatic nerve , neurotrophic factors , peripheral nerve injury , nerve guidance conduit , regeneration (biology) , neuroscience , peripheral nervous system , brain derived neurotrophic factor , low affinity nerve growth factor receptor , nerve injury , medicine , anatomy , biology , central nervous system , microbiology and biotechnology , receptor
J. Neurochem. (2012) 122 , 501–511. Abstract Up‐regulation of neurotrophin synthesis is an important mechanism of peripheral nerve regeneration after injury. Neurotrophin expression is regulated by a complex series of events including cell interactions and multiple molecular stimuli. We have studied neurotrophin synthesis at 2 weeks time‐point in a transvertebral model of unilateral or bilateral transection of sciatic nerve in rats. We have found that unilateral sciatic nerve transection results in the elevation of nerve growth factor (NGF) and NT‐3, but not glial cell‐line derived neurotrophic factor or brain‐derived neural factor, in the uninjured nerve on the contralateral side, commonly considered as a control. Bilateral transection further increased NGF but not other neurotrophins in the nerve segment distal to the transection site, as compared to the unilateral injury. To further investigate the distinct role of NGF in regeneration and its potential for peripheral nerve repair, we transduced isogeneic Schwann cells with NGF‐encoding lentivirus and transplanted the over‐expressing cells into the distal segment of a transected nerve. Axonal regeneration was studied at 2 weeks time‐point using pan‐neuronal marker NF‐200 and found to directly correlate with NGF levels in the regenerating nerve.