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Stimulation of GluN receptors decreases the surface density of GluN1/GluN2B subunits in cultured neocortical interneurons
Author(s) -
Nörenberg Wolfgang,
Lindemeyer A. Kerstin,
Wilmes Thomas,
Sobottka Helga,
Meyer Dieter K.
Publication year - 2012
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2012.07718.x
Subject(s) - nmda receptor , receptor , stimulation , neocortex , neuroscience , neurotoxicity , protein subunit , chemistry , long term depression , biology , glutamate receptor , biophysics , microbiology and biotechnology , biochemistry , ampa receptor , toxicity , organic chemistry , gene
J. Neurochem. (2012) 121 , 587–596. Abstract Changes in the density of NMDA (GluN) receptors in the neuronal membrane are critical for plasticity, whereas malfunction of precisely regulated GluN receptor activity may be involved in neurotoxicity. In cultured rat neocortical interneurons, we have studied the regulation of the surface density of GluN1, GluN2A and GluN2B subunits. Application of 5 μMol NMDA for 24 h followed by a washout period of 24 h decreased the response of GluN receptors for at least 2 days. The reduction was caused by a decrease in the surface density of GluN1/GluN2B subunits, whereas GluN2A subunits remained unaffected. Our data indicate that long but reversible low level activation of GluN receptors can cause long‐term changes in their subunit composition in cultured interneurons.