Premium
Acute administration of fluoxetine normalizes rapid eye movement sleep abnormality, but not depressive behaviors in olfactory bulbectomized rats
Author(s) -
Wang YiQun,
Tu ZhiCai,
Xu XingYuan,
Li Rui,
Qu WeiMin,
Urade Yoshihiro,
Huang ZhiLi
Publication year - 2012
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2011.07558.x
Subject(s) - fluoxetine , monoamine neurotransmitter , open field , rapid eye movement sleep , medicine , endocrinology , psychology , serotonin , slow wave sleep , sleep onset , sleep (system call) , midbrain , behavioural despair test , hippocampus , neuroscience , eye movement , central nervous system , psychiatry , antidepressant , insomnia , electroencephalography , receptor , computer science , operating system
J. Neurochem. (2012) 120 , 314–324. Abstract In humans, depression is associated with altered rapid eye movement (REM) sleep. However, the exact nature of the relationship between depressive behaviors and sleep abnormalities is debated. In this study, bilateral olfactory bulbectomy (OBX) was carried out to create a model of depression in rats. The sleep–wake profiles were assayed using a cutting‐edge sleep bioassay system, and depressive behaviors were evaluated by open field and forced swimming tests. The monoamine content and monoamine metabolite levels in the brain were determined by a HPLC‐electrochemical detection system. OBX rats exhibited a significant increase in REM sleep, especially between 15:00 and 18:00 hours during the light period. Acute treatment with fluoxetine (10 mg/kg, i.p.) immediately abolished the OBX‐induced increase in REM sleep, but hyperactivity in the open field test and the time spent immobile in the forced swimming test remained unchanged. Neurochemistry studies revealed that acute administration of fluoxetine increased serotonin (5‐HT) levels in the hippocampus, thalamus, and midbrain and decreased levels of the 5‐HT metabolite 5‐hydroxyindoleacetic acid (5‐HIAA). The ratio of 5‐HIAA to 5‐HT decreased in almost all regions of the brain. These results indicate that acute administration of fluoxetine can reduce the increase in REM sleep but does not change the depressive behaviors in OBX rats, suggesting that there was no causality between REM sleep abnormalities and depressive behaviors in OBX rats.