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Cerebral ischemic pre‐conditioning enhances the binding characteristics and glutamate uptake of glial glutamate transporter‐1 in hippocampal CA1 subfield of rats
Author(s) -
Liu AiJu,
Hu YuYan,
Li WenBin,
Xu Jing,
Zhang Min
Publication year - 2011
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2011.07396.x
Subject(s) - glutamate receptor , hippocampus , hippocampal formation , transporter , glutamate aspartate transporter , neuroscience , ischemia , chemistry , pharmacology , excitatory amino acid transporter , biology , biochemistry , microbiology and biotechnology , medicine , receptor , gene
J. Neurochem. (2011) 119 , 202–209. Abstract Glial glutamate transporter‐1 (GLT‐1) is the predominant subtype of glutamate transporters which are responsible for the homeostasis of extracellular glutamate. Our previous studies have shown that up‐regulation in GLT‐1 protein expression matches brain ischemic tolerance induced by cerebral ischemic preconditioning (CIP). To specify the role of functional changes of GLT‐1 in the induction of brain ischemic tolerance by CIP, the present study was undertaken to examine changes in the binding properties of GLT‐1 (including maximum binding and affinity for glutamate) and in GLT‐1 mediated glutamate uptake, using L‐ 3 H‐glutamate assay in the rat hippocampus. The results indicated that CIP was able to increase the maximum binding and affinity, and uptake of GLT‐1 for glutamate in hippocampal CA1 subfield either with or without the presence of the subsequent severe brain ischemic insult. Simultaneously, accompanied with the above changes, CIP significantly reduced the delayed neuronal death (DND) in this region induced by lethal global cerebral ischemia. It could be concluded that up‐regulation in the maximum binding and affinity and glutamate uptake of GLT‐1 contributed to the neuronal protection of CIP against global cerebral ischemic insult.