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Characterization of Dp71Δ 78‐79 , a novel dystrophin mutant that stimulates PC12 cell differentiation
Author(s) -
Aragón Jorge,
RomoYáñez José,
MartínezHerrera Alejandro,
Ceja Víctor,
Rendon Alvaro,
Montañez Cecilia
Publication year - 2011
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2011.07347.x
Subject(s) - neurite , microbiology and biotechnology , transfection , biology , cellular differentiation , cell culture , enolase , immunology , in vitro , gene , immunohistochemistry , genetics
J. Neurochem. (2011) 119 , 697–707. Abstract Dp71 has an important role in the central nervous system. To better understand the function of Dp71 domains in neuronal differentiation, PC12 cells were stably transfected with a dystrophin mutant, Dp71Δ 78‐79 , which lacks exons 78 and 79. Based on the percentage of cells bearing neurites and neurite length analyses, we found that cells stably expressing Dp71Δ 78‐79 (PC12‐C11) differentiate more efficiently than non‐transfected cells. While wild‐type cells reach their maximum differentiation 9–12 days after initiating the differentiation process, the PC12‐C11 cells reach differentiation in 4–6 days. Protein expression analysis showed a down‐regulation of Dp71a and an up‐regulation of Dp71ab and/or Up71, β‐dystroglycan and neuron‐specific enolase in undifferentiated and in neural growth factor differentiated PC12‐C11 cells. No change was observed in the expression of Grb2 and Up400. The subcellular localization of Dp71Δ 78‐79 was in the cell periphery, and there was no change in localization during the differentiation process, which was also observed throughout the neurite extensions.