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Methamphetamine oxidatively damages parkin and decreases the activity of 26S proteasome in vivo
Author(s) -
Moszczynska Anna,
Yamamoto Bryan K.
Publication year - 2011
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.2010.07147.x
Subject(s) - parkin , meth , methamphetamine , proteasome , neurodegeneration , oxidative stress , neurotoxicity , ubiquitin , pharmacology , dopaminergic , chemistry , biochemistry , biology , dopamine , toxicity , parkinson's disease , medicine , endocrinology , disease , monomer , organic chemistry , acrylate , gene , polymer
J. Neurochem . (2011) 116 , 1005–1017. Abstract Methamphetamine (METH) is toxic to dopaminergic (DAergic) terminals in animals and humans. An early event in METH neurotoxicity is an oxidative stress followed by damage to proteins and lipids. The removal of damaged proteins is accomplished by the ubiquitin‐proteasome system (UPS) and the impairment of this system can cause neurodegeneration. Whether dysfunction of the UPS contributes to METH toxicity to DAergic terminals has not been determined. The present investigation examined the effects of METH on functions of parkin and proteasome in rat striatal synaptosomes. METH rapidly modified parkin via conjugation with 4‐hydroxy‐2‐nonenal (4‐HNE) to decrease parkin levels and decreased the activity of the 26S proteasome while simultaneously increasing chymotrypsin‐like activity and 20S proteasome levels. Prior injections of vitamin E diminished METH‐induced changes to parkin and the 26S proteasome as well as long‐term decreases in DA and its metabolites’ concentrations in striatal tissue. These results suggest that METH causes lipid peroxidation‐mediated damage to parkin and the 26S proteasome. As the changes in parkin and 26S occur before the sustained deficits in DAergic markers, an early loss of UPS function may be important in mediating the long‐term degeneration of striatal DAergic terminals via toxic accumulation of parkin substrates and damaged proteins.